Water Nuggets Practice Exam 2025 – Complete Study Resource

Question: 1 / 400

In the MNO-MUG test, E.coli presence is indicated by what color change?

Red or Pink Color

Blue Fluorescence

In the MNO-MUG test, the presence of E. coli is indicated by blue fluorescence. This method relies on the detection of the enzyme β-glucuronidase, which is produced by E. coli. When the substrate MUG (4-methylumbelliferyl-β-D-glucuronide) is hydrolyzed by this enzyme, it releases a compound that fluoresces blue under ultraviolet light. This characteristic response is a reliable indicator of E. coli contamination in a sample.

The other options, although they may relate to different tests or indicators, do not specifically correspond to the MNO-MUG test's mechanism for detecting E. coli. Understanding the specific biochemical reactions involved helps in accurately interpreting test results.

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Meter Count

Green Color

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